LOCUS       284_TagRFPT_C1_S       10497 bp ds-DNA     circular     03-JUN-2015
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and SpeI to fuse 5' homology arm directly to
            mKate2, or 
COMMENT     -Digest with AvrII and SpeI to include a flexible linker (for
            C-terminal tags).
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    398..1010
                     /label=This sequence will be replaced by the homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2788..2928,2976..3809)
                     /label=SQT-1
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6118..7143
                     /note="hygromycinR"
                     /label=hygR
                     /ApEinfo_fwdcolor=#006666
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2004..2033
                     /label=myc
                     /ApEinfo_fwdcolor=#6666ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(4182..4423,4475..4845,4897..5312))
                     /label=Cre
                     /ApEinfo_fwdcolor=#6699cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    complement(8374..8386)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
                     /label=CAP protein binding site
                     /ApEinfo_fwdcolor=#990099
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        complement(8678..9230)
                     /note="RNAII transcript (complementary strand)"
                     /label=RNAII transcript (complementary strand)
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8678..9266)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        9120..9227
                     /note="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1961..1994
                     /label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1995..2000
                     /label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8660..9342)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(9240..9245)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9440..10099)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      9084..9089
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label=RNAI promoter (clockwise); TTGAAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      9106..9111
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label=RNAI promoter (clockwise); GCTACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     5'UTR           join(2702..2729,2786..2787)
                     /label=sqt-1 5'UTR
                     /ApEinfo_fwdcolor=#ccffcc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9437..10297)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1950..1960
                     /label=intron(1)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1038..1181,1233..1460,1512..1694,1746..1922)
                     /label=TagRFP-T
                     /ApEinfo_fwdcolor=#ff3300
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3039..3039
                     /label=e1350
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2001..2003
                     /label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(9261..9266)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           3810..3846
                     /label=sqt-1 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7627..7653
                     /label=Flag
                     /ApEinfo_fwdcolor=#ff6699
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2037..2417
                     /label=let-858 Terminator
                     /ApEinfo_fwdcolor=#cc0000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8272..8292)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7600..7626
                     /label=Flag(1)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1923..1943
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7561..7572
                     /label=spacer(1)
                     /ApEinfo_label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7573..7599
                     /label=Flag(2)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#cc3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             754..963
                     /label=ccdB(1)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5313..5712)
                     /label=hsp16-41 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           7169..7520
                     /label=unc-54 3'UTR
                     /ApEinfo_fwdcolor=#990000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           complement(3847..4178)
                     /label=tbb-2 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#993333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8261..8267
                     /label=This sequence will be deleted if ClaI is used
                     /ApEinfo_fwdcolor=#ffcc99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2418..2787
                     /label=Psqt1
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7654..8260
                     /label=This sequence will be replaced by the homology
                     arm(1)
                     /ApEinfo_label=This sequence will be replaced by the
                     homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          7555..7560
                     /label=intron(2)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7698..8003)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB(2)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7521..7554
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(7701..7910)
                     /label=ccdB(3)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5713..6075
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /label=Prps-0
                     /ApEinfo_fwdcolor=#339933
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG GTCTCCAAGG GAGAGGAGCT CATCAAGGAG AACATGCACA
     1081 TGAAGCTCTA CATGGAGGGA ACCGTCAACA ACCACCACTT CAAGTGCACC TCCGAGGGAG
     1141 AGGGAAAGCC ATACGAGGGA ACCCAAACCA TGCGTATCAA Ggtaagttta aacatatata
     1201 tactaactaa ccctgattat ttaaattttc agGTCGTCGA GGGAGGACCA CTCCCATTCG
     1261 CCTTCGACAT CCTCGCCACC TCCTTCATGT ACGGATCCCG TACCTTCATC AACCACACCC
     1321 AAGGAATCCC AGACTTCTTC AAGCAATCCT TCCCAGAGGG ATTCACCTGG GAGCGTGTCA
     1381 CCACCTACGA GGACGGAGGA GTCCTCACCG CCACCCAAGA CACCTCCCTC CAAGACGGAT
     1441 GCCTCATCTA CAACGTCAAG gtaagtttaa acagttcggt actaactaac catacatatt
     1501 taaattttca gATCCGTGGA GTCAACTTCC CATCCAACGG ACCAGTCATG CAAAAGAAGA
     1561 CCCTCGGATG GGAGGCCAAC ACCGAGATGC TCTACCCAGC CGACGGAGGA CTCGAGGGAC
     1621 GTACCGACAT GGCCCTCAAG CTCGTCGGAG GAGGACACCT CATCTGCAAC TTCAAGACCA
     1681 CCTACCGTTC CAAGgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1741 ttcagAAGCC AGCCAAGAAC CTCAAGATGC CAGGAGTCTA CTACGTCGAC CACCGTCTCG
     1801 AGCGTATCAA GGAGGCCGAC AAGGAGACCT ACGTCGAGCA ACACGAGGTC GCCGTCGCCC
     1861 GTTACTGCGA CCTCCCATCC AAGCTCGGAC ACAAGCTCAA CGGAATGGAC GAGCTCTACA
     1921 AGGAGAATCT GTACTTTCAA TCCGGAAAGg taagtttaaa ATAACTTCGT ATAGCATACA
     1981 TTATACGAAG TTATtttcag GGAGAGCAAA AGCTTATCTC CGAGGAGGAC CTCTAAGGAT
     2041 GATCGACGCC aACGTCGTTG AATTTTCAAA TTTTAAATAC TGAATATTTG TTTTTTTTCC
     2101 TATTATTTAT TTATTCTCTT TGTGTTTTTT TTCTTGCTTT CTAAAAAATT AATTCAATCC
     2161 AAATCTAAac attttttttt ctctttccgt ctcccaattc gtattccgct cctctcatct
     2221 gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac gtggggggaa
     2281 ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac acacaatatg
     2341 caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa aaatatatag
     2401 aggaggaagg aaactagctg ccctcgattg tctatcttcc attctcacca agtttcaaca
     2461 actttattca gtcattcttc tctggttcat ttcccaacca atcttcccga ataaagccca
     2521 tctgtccttt cggccacatt cctcacctac atccgtactc cttatctccc gttcttatcc
     2581 agtcttttat cgtctctgcg tctcgcacac tgtctcgtcg gtaatgtaaa aaaaaaacaa
     2641 aaaaaacttg aaaacttgcg gaaagatttc ctaataaaag gagacgaatc cggtaggaaa
     2701 tcgacaagtc tctgcttctc gaagactagg tgagttacat ttctttggcc gaattatttt
     2761 gaaatcctaa atttgataat ttcagagATG TCTGTAAAAC TTGCGTGTTA TGTGACGGCT
     2821 TCGGTCACTG TCGCCACTCT TATGGTATGC TTCATGACCA TGTCAACCAT CTACTCGGAG
     2881 GTTGATGGAT TCAGAGAGAA GCTTGATACC GAGATGAATG TGTTCAGAgt gagttaaatt
     2941 atattttgaa ttttaaataa tttttaattt tctagCAATC TACCAATGGA TTGTGGAAGG
     3001 ACATAGTTGT CATCGGAAGA TCTAGCAAGC GTGTCCGTTG TCAATATGAA GAGACCAACG
     3061 CTACCCCAAC TCCACATGCT GATGGATCCC CATCTGCTCC ACCAGGTCAA CCACCAGCAG
     3121 TTCCACCAGT CTTCAACCAG CCAAAGACTC CAAATGGAGC CAATGGAAAT GGACCAACCT
     3181 GCAACTGCAA TGCTGATAAC AAGTGCCCAG CTGGACCATC CGGACCAAAG GGAGTTCCAG
     3241 GAGTTCCAGG ACTCGACGGA GTTCCAGGAC TTGACGGTGT TCCAGGAGTT GGAGCTGATG
     3301 ATATCGCTCC ACAACGCGAG TCTGTCGGAT GCTTCACTTG CCCACAAGGA CCAGTTGGAC
     3361 CACCAGGAGC TCTTGGAAGA CCAGGACCAC GTGGACTTCC AGGACCAAGA GGACAAAATG
     3421 GAAACCCAGG AAGAGATGGA CAACCAGGAC ATCCAGGAGA GCAAGGATCA TCCGGTCAAA
     3481 TCGGAAAGAT CGGAGAGCCA GGACCACCAG GAGAGAAGGG ACGCGACGCC GAGCATCCAA
     3541 TCGGAAGACC AGGACCAAAG GGACCAAGAG GAGATCAAGG ACCAACAGGA CCAGCTGGAC
     3601 AGAACGGTCT TCACGGACCA CCAGGAGAGC CAGGAACCGT TGGACCAGAA GGACCATCTG
     3661 GAAAGCAAGG ACGTCAAGGA CCAGACGGAA CCCAGGGAGA GACTGGACCA GACGGAAGAC
     3721 CAGGAAAGGA TGCCGAGTAC TGCCAGTGCC CAGACAAGTC TCCACCATCA GAGGCTGTCA
     3781 ACGCCAACCG TGGATACAGA AATATCTAAa ttgttggtgt tttctaataa aatatttgag
     3841 attcagtgag acttttttct tggcggcaca ataaagtttc ctctttttgt tgggtcactc
     3901 aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg ggtttaaagc
     3961 acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc acattttgat
     4021 agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat aaaataataa
     4081 cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag aagagtgata
     4141 gagaagaagg gaatgcttga aaggattttg catttatctt aATCACCGTC TTCGAGAAGA
     4201 CGAACCATGG CGCCAGTTTC AGAATCCAAG TTTCTAATAT AGTTCATCAC AATGTTGACG
     4261 TTGGTCCAAC CTCCGGCTTG CATAATCTCT GGGATGGAAA CTCCAGCGCG AGCCATGTCA
     4321 CGGGCAGCTC CGACACGGGC GGAATGTCCA GACCAAGCCA AGTAACGTTG TCCGGAATCA
     4381 TCCTTGGCTC CGTAGATGAG ACGGTGGGTA GCTTCGAAGA TTCctgaaaa tttattttca
     4441 gcttattctt agctaatatg ttgtttaaac ttacCTTCAA GAGCTCGAGT CGAGAGTTGT
     4501 GATGTTGCAC TCGGGGCAGC AACTCCGTTC TTGCGAACAC GACAGAACAG GTAGTTGTTT
     4561 GGATCATCGG CGACTCCGGA AACCGAAATC CAGCGCTCAA CGAGCTTGGT AACTCCAAGG
     4621 GAGAGCGCTT TTTCGACTCC GGCAGTCGAG ACCAGCGTTT TTGTACGTCC GATGTGGATG
     4681 AGCATTCTTC CTCCGTCTGT GCGACTAATA TCCTTGACGC GAATGCGGGC AATTTCGGCG
     4741 ATACGGAGAA GTGTGTTGTA AGCAATTCCG AGGAAGGCGA GGTTTCGGAT GTCCTGGCAA
     4801 CGATCGGAAT TTTCCATAAG AGAACGGACC TGATCGAAGT CGGTTctgaa aatttcgtca
     4861 agtcttagga ttgaatacat taatatttaa acttacCTTT CGAAGGCGAG GGCTTGCTTG
     4921 GCTCTCTCTC CAGCATCAAC ATTTTCCTTG CGGATACGTC TCATTACGAG AGATACGGCA
     4981 TTAGAATCTG ATGGGCGTGG AAGTCCAGAT CGGCGATGGA GCATATTGAG CTGTCCGAGA
     5041 TGTTGTTGGA TCGTCTTCAC CGCCAATCCA CGAGCTTGGA GGTAGAGCAA GTAGTCACGA
     5101 ACGTCTTCTG GCTCGGCTGG GAACCACTTG CGGTTGTTAA GCTTGCACCA GGCAGCCCAA
     5161 GAACGGCACA CTGAGAGAAG CATCTTCCAG GTGTGCTCGG AGAAGGCTTG TCTGTCACGG
     5221 AACATATCCA TGAGGTTCTT GCGAACTTCA TCACTTGTCG CGTCAACTGG AAGTGCTGGA
     5281 AGATTTTGAT GAACAGTGAG AAGATTTGAC ATattttcga agttttttag atgcactaga
     5341 acaaagcgtg ttggcttcct ctgagcccgc tttccttata tacccgcatt ctgcagcctt
     5401 acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa atactcccgg tgggtgcaaa
     5461 gagacgcaga cggaaaatgt atctgggtct ctttattgtg tacactactt ttccatgtac
     5521 cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa tgttctagaa aaaggtggaa
     5581 aatagtataa ataccgttga aaataaatac cgaacaacat ttgctctaat tgtgaaatta
     5641 gaaatcttca aactataatc atgtcacttt accactattt ccgtccagct caacgttccg
     5701 tttttggtga tcATTTTTGC TTTCGTCGTA AATCTACACA CGCGTCTCTT CCGTGCGAGA
     5761 GTCCAAGCCA GCAGCCAAAT TCGTTGACTG AGTATTCAAC GTTTATACGT TGTCGGCAAC
     5821 GAGAAATAGG AAAATGCATC GGGAAATGTT CTTTTTTCGA TTTTTTCCAA GGTTTTGACA
     5881 AATTTTACCA CGAATTTTGC TATGTTTTCA ATTAAAAAAT ATGTTATTCA ACTGTTTCTA
     5941 TGAGGAAAAT AAGGCTTTGC ATGTAATTTT CTTATTCAGC ATAATTTTTA ATTAATTTGA
     6001 ATTTTCTGTC CTAACGTTTA TTTTGTTTTC TTGGTTATGA CTGATCTGAA ATTAATTTTT
     6061 GAATTTTAAG GTAATATGTC AGGCGGTGCC GCAAGTTTGT ACAAAAAAGC AGGCTCCATG
     6121 AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC TGATCGAAAA GTTCGACAGC
     6181 GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC GTGCTTTCAG CTTCGATGTA
     6241 GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT
     6301 TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG
     6361 GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG CACAGGGTGT CACGTTGCAA
     6421 GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG TCGCGGAGGC CATGGATGCG
     6481 ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC CATTCGGACC GCAAGGAATC
     6541 GGTCAATACA CTACATGGCG TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC
     6601 TGGCAAACTG TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG
     6661 ATGCTTTGGG CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA TTTCGGCTCC
     6721 AACAATGTCC TGACGGACAA TGGCCGCATA ACAGCGGTCA TTGACTGGAG CGAGGCGATG
     6781 TTCGGGGATT CCCAATACGA GGTCGCCAAC ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT
     6841 ATGGAGCAGC AGACGCGCTA CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCGCGG
     6901 CTCCGGGCGT ATATGCTCCG CATTGGTCTT GACCAACTCT ATCAGAGCTT GGTTGACGGC
     6961 AATTTCGATG ATGCAGCTTG GGCGCAGGGT CGATGCGACG CAATCGTCCG ATCCGGAGCC
     7021 GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT
     7081 GTAGAAGTAC TCGCCGATAG TGGAAACCGA CGCCCCAGCA CTCGTCCGAG GGCAAAGGAA
     7141 TAGACCCAGC TTTCTTGTAC AAAGTGGGTC CAATTACTCT TCAACATCCC TACATGCTCT
     7201 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT
     7261 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA
     7321 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC
     7381 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT
     7441 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA
     7501 GTTTATGACC GCAATTTTTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATtttcag
     7561 GGAGCCGGAT CTGATTATAA AGACGATGAC GATAAGCGTG ACTACAAGGA CGACGACGAC
     7621 AAGCGTGATT ACAAGGATGA CGATGACAAG AGActagtTC GACCTGCAGA CTGGCTGTGT
     7681 ATAAGGGAGC CTGACATTTA TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA
     7741 TTTTCGCGGT GGCTGAGATC AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC
     7801 ATATCGGTGG TCATCATGCG CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA
     7861 CGGGGGACTT TATCTGACAG CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG
     7921 GGCGTGTCAA TAATATCACT CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA
     7981 TAGGTGTAAA CCTTAAACTG CATTTCACCA GCCCCTGTTC TCGTCGGCAA AAGAGCCGTT
     8041 CATTTCAATA AACCGGGCGA CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC
     8101 GGCACGCAGA CGACGGGCTT CATTCTGCAT GGTTGTGCTT ACCGAACCGG AGATATTGAC
     8161 ATCATATATG CCTTGAGCAA CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC
     8221 TTCATAGCAT ACCTCTTTTT GACATACTTC GGGTtcctag gaaatcgata acatggtcat
     8281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
